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Histone h3 glutathionylation in proliferating mammalian cells destabilizes nucleosomal structure

PfGrx-dependent reduction kinetics for roGFP2 WT (S 2 ) To address the previously suggested alternative models for the glutaredoxin-dependent reduction of non-glutathione protein disulfides (Fig

The plate was then incubated at 37 C for 45 minutes in a shaker water bath then cooled and the absorbance of the pink colored chromophore was measured at 540 nm using ELISA reader, against a blank treated in a similar manner to the test but using 100l distilled water instead of the sample