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Blood Sample Preparation

In the first step, cysteine and glutamate are linked in a reaction catalyzed by the -glutamylcysteine synthase (-GCS) to form -glutamylcysteine

2) and the MM-like expression derived from our formulation clarify that phosphorylation and -arrestin binding are the two main processes responsible for shaping a drug tolerance (a suppressed downstream signaling at high agonist concentration), a dose-response widely observed in biochemical assays (Fig