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To investigate how metformin exerted its antioxidant effects, we first examined the expression of Nrf2 and its downstream regulatory protein HO-1 in the chondrocytes exposed to H 2 O 2 , with or without metformin treatment

after stimulation of SMase-dependent ceramide formation, assembly of the death-inducing signaling complex (DISC) and oligomerization of Fas receptor, as well as cleaved caspase-8 and caspase-3, were detected [130]

Thats a reasonable starting assumption, but it misses a few important considerations specific to this peptide