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To address this knowledge gap, we have developed a series of genetic models that permit spatiotemporal control over the expression of GCLC, the rate-limiting enzyme in GSH synthesis
subtilis rib operon consists of five genes, ribDG, ribE, ribAB, ribH, ribT , forming one transcription unit (mRNA), and contains the regulatory region ribO , untranslated leader region, that is located with the major promoter P1 (transcription start) upstream of the first structural gene in the operon (Figure 2) (Sklyarova et al., 2012)
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