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This includes the downregulation of Bax (a pro-apoptotic marker), upregulation of Bcl-2 (an anti-apoptotic protein), and increased expression of proliferating cell nuclear antigen (PCNA), a key marker of cell proliferation [65]

When discontinued, the levels of HGH slowly resume normalcy, which may cause a gradual reversion of either of the following effects

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Establishment of K7M2-Luc orthotopic osteosarcoma mouse model and therapeutic effect evaluation Tumor model establishment To establish orthotopic model, K7M2 cells (3 10 6 cells/well) were dispersed in PBS buffer and implanted into right tibia of BALB/c mice