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Step 6: Transfer the reconstituted solution to pre-chilled, sterile low-protein-binding microcentrifuge tubes for aliquoting, minimizing repeated freeze-thaw cycles during subsequent research applications

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Interestingly, when ghrelin is administered alone to cells coexpressing D1R and GHSR1a, no increase in cAMP accumulation is observed, while typical levels of Ca 2+ accumulation associated with GHSR1a activation occur (Jiang et al., 2006)