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A benefit of using biotin as an The affinity tag is that it has little effect on due to its small size, the activity of a large biomolecule (244Da).Streptavidin is a large protein (60 kDa) that can be obtained from Streptomyces avidinii and have an affinity constant of 1013 M1 for biotin

Some GPxs are therefore dependent on Se and use GSH as a reducing agent, while others, called TGPx, do not contain Se (NS-GPx) and reduce ROS using thioredoxin, which acts as ROS sensors in various pathways and signal transduction

In vitro colonic fermentation of dietary fibers: fermentation rate, short-chain fatty acid production and changes in microbiota