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Targeted xCT-mediated ferroptosis and protumoral polarization of macrophages is effective against HCC and enhances the efficacy of the anti-PD-1/L1 response

In addition, the characterized LASSS treatment did not stimulate autolysis of HGF as monitored by SDS-PAGE for degradation of 60-kDa -chain and 90-kDa proform of recombinant HGF incubated with LASSS (at 1:8000) at 25 for 2 h (see supplementary Figure 1b), providing another important basis for the availability of LASSS-HGF

Previous studies indicated that miRNAs are essential targets for curcumin, and curcumin mediates its curative effects (Mirzaei et al., 2016b

Freeze-thaw cycles: if a validated frozen stock is used, make small aliquots and avoid repeated thawing