You have to ignore the idea that you are upsetting the doctor, or that you are insulting them by questioning poor decisions about your health and wellbeing
The following reaction system was used: 1 ng pGEX4T-2 plasmid, 50 mM Tris-HCl (pH 7.8), 5 mM MgCl 2 , 10 mM 2-mercaptoethanol, 10 g/ml BSA, 1 ng forward primer FP1 and 1 ng reverse primer RP1, 20 M dNTP, 1 M dideoxynucleotides (ddNTP), 2 units DNA Polymerase I (Invitrogen), and ddH 2 O to the reaction volume of 100 l
However, most subsequent clinical research has focused on androgenetic alopecia specifically, so the strongest evidence base is for pattern hair loss
This is convenient for patients who prefer fewer injections compared to daily treatments like liraglutide (Victoza)