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They'd get a cheap victory

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Kumar S, Stecher G, Li M, Knyaz C, Tamura K

A consistent column temperature of 25 C was maintained throughout the gradient program, which started with 0.5% B for the first minute, increased to 80% B at 10 min, reached 100% B at 15 min with a hold until 17.5 min, and then reverted to 0.5% B, holding until 20 min