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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

PBS vehicle 10 -12 M Nlel-AnglV as a positive control DVI12 various concentrations for several stabilized analogs for 5 days in culture or for 30 minutes prior to fixation on day in vitro 12 A acetyl- Nle-YIH B D-NleOYI C GABA-YI FIG

Groundwater in the Region is generally of high quality, but faces numerous threats including, industrial spills, leaking underground storage tanks, improperly maintained septic systems, urban runoff, and inefficient agricultural operations

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