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In contrast to sub-clinical inflammation in the skin of reactive patients, expression of metallothioneins was associated with protection from HDM-driven inflammatory reaction

Following permeabilization, the muscle fiber bundles were washed with buffer Z [105 mM K-MES, 30 mM KCl, 10 mM K 2 HPO 4 , 5 mM MgCl 2 6H 2 O, 0.5 mg/mL BSA, and 1 mM EGTA, pH 7.4] with 0.5 mM pyruvate and 0.2 mM malate for 15 min at 4C

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