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The precipitated pellet was then mixed with sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) sample loading buffer and analyzed by immunoblotting

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Status asthmaticus: Intensive bronchodilation (continuous albuterol nebulization, IV magnesium sulfate 2 g over 20 min), systemic corticosteroids (IV methylprednisolone J2210), supplemental O (SpO target 9395%), monitoring for respiratory failure