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A few things had been added to the essential core: a sound reader, a chassis of sorts, take-up arms and a cloth bin attached to the rear of the chassis to keep film from spilling onto the floor

hLMVECs were first seeded on the basolateral side of each membrane in the AX12 at a density of 106,000cells/cm 2 , followed by a 2-h incubation, and then AX iAECs were seeded apically at a density of 409,000 cells/cm 2 on day 0 for all co-culture experiments

Jokingly hand them a cigarette or smoke in front of them