Crosslinking mass spectrometry (XL-MS) The final concentration of 0.5 M DDD-Ube2e2-COP1-c-Jun was incubated with a final concentration of 0.1 mM DSBU crosslinker (Thermo Fisher, A35459) in the wash buffer (20 mM HEPES-NaOH pH 7.4, 200 mM NaCl, 2 mM MgCl 2 , 0.5 mM TCEP) for 40 min at 25 C and quenched with 10 mM Tris-HCl pH 8.0 for 10 min at 25 C
Possible side effects include mild headache, injection site pain, temporary nausea, low blood pressure, or, rarely, allergic reactions and infections
Pregnancy complications and birth weights were measured but showed no significant results between the IF-treated group and the controlled group (30)
Adult cartilage shows declining cellularity, accumulated matrix damage, reduced proteoglycan content, calcification advancement