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Fixed tissues were routinely processed, embedded in paraffin, sectioned at a thickness of 3 m, and were stained with hematoxylin and eosin (HE) for histopathological examination and with Perls Prussian blue for detection of iron accumulation

In another study conducted in a rat model of metabolic syndrome, chronic treatment with liraglutide showed a greater increase in coronary vessel internal diameter than vehicle control (Sukumaran et al., 2020)

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They ensure that serious conditions are identified early and that investigations are appropriately directed