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For this reason, the presence of serum in the media during the experiment has many disadvantages and may lead to serious interpretive errors when analyzing the results, as these results depend on too many variables which are out of control
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Briefly, we quantified GPs using the label-free, intensity-based absolute quantification (iBAQ) method and then normalized to final quantificational value (FOT) defined as the iBAQ value of an individual protein divided by the total iBAQ values of all identified proteins within one experiment
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