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Hierarchical clustering was performed using the Morpheus Software ( with the Pearson correlation and average linkage method

The two primary MTHFR variants are C677T (rs1801133) which substitutes valine for alanine at position 222, reducing enzyme activity by approximately 35% in heterozygotes and 70% in T/T homozygotes at physiological folate concentrations and A1298C (rs1801131) which substitutes glutamate for alanine at position 429, reducing activity by approximately 20% in heterozygotes, with compound heterozygosity (C677T + A1298C) producing effects between the two individual homozygous states

Behne, D., Kyriakopoulos, A., Meinhold, H

10 Hydroxocobalamin, Methylcobalamin and L-Methylfolate for MTHFR Methylcobalamin is the most readily available and intracellularly active form of B12, and is relatively easy to find in supplements