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In a landmark study from Edward Marcottes laboratory, the swappability of 414 essential genes in yeast was tested by replacement with their human orthologs

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(1) V Kh Khavinson, I E Bondarev, and A A Butyugov (2003) Epithalon peptide induces telomerase activity and telomere elongation in human somatic cells Bulletin of Experimental Biology & Medicine, 2003 Jun, Volume 135 (Issue 6), Pages 590-2

The coding sequence of full-length potential interactors was cloned into the prey vector pGADT7 and co-transformed with pGBKT7-SsCm1 SP into Y2HGold yeast cells