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The data indicated that UHPSFC/MS shows promise in qualitative metabolomics analysis but requires further improvement, such as optimizing sample preparation to develop sensitive quantitative methods using UHPSFC/MS

Recent methodological advancesincluding organotypic slice cultures, three-dimensional brain organoids and in vivo imaging approachesoffer scalable and physiologically relevant platforms that more faithfully capture astrocyte heterogeneity and functional consequences

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