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Description

Cells were collected with PBS 1x -EDTA 2 mM, centrifuged at 1200 rpm for 3 min, re-suspended and incubated with 1 L of CD133 (AC133-PE human, Miltenyi Biotec) and 99 L PBS-BSA 0.1% for 20 min in the dark 4 C

Ethics statement All animal experiments were conducted in accordance with applicable laws and institutional guidelines, approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Brain Education