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DNA dot blot assay The gDNA methylation level was detected using a dot blot assay as described previously with modifications 49

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30 (2), e14576

The following validated biomarkers offer clear insight into these peptide-linked responses: Collagen type I and III levels: These markers are measured through fluorescence or staining methods and indicate structural changes in the extracellular matrix, offering a reliable way to track collagen deposition and remodelling throughout controlled laboratory experiments